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Hi Treangen, Thank you very much for the software. I have no difficulties in genomes alignment of a small sample sizes (up to 45 bacterial genomes). But I am faced with a problem for the sample size o…
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Hi, Felix.
I'm so sorry to bother you again. I have WGBS data from the same batch for two species (Accel Swift data) and have already trimmed it using the method you suggested, with the following c…
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Hii,
I used STAR aligner to map reads to potato and pepper genome. I found that the mapping time for pepper was around 3-4 hours with SE (30 million reads) sample on pepper genome of 3GB in size. W…
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Let's say I have four genomes: A, B, C and D with tree (((A, B), C), D), and there is a gene "X" I'm interested in that is present in A, B and D, but deleted in genome C. Am I correct that the default…
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It seems, graphmap have some problem with "align" flag. How to fix it ?
➜ bin git:(master) ✗ ~/Tools/EAGLER/release/eagler -x ont -t 40 /media/urbe/TOSHIBALAB/falconUnzip/SSPACED_P2/scaffolds.fas…
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Hi,
I have been trying to run the chip-seq workflow of seq2science. It starts but stops when 7% of the jobs are done.
```
seq2science --version
seq2science: v0.5.1
```
**To Reproduce**
Plea…
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I have to annotate novel diplonema genome. This species contains non-cannonical intron (Non GT-AG). So that I chose to use braker.
I have run command prothint to generate and prothint_augustus.gff…
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Hi there,
I'd like to try using CIRCexplorer2 to map circRNA reads in human genome. But since my project has over 1000 samples and we've already done the alignment work for mRNA by STAR aligner (whic…
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Hi there,
I'd like to try using CIRI2 or CIRIquant to map circRNA reads in human genome. But since my project has over 1000 samples and we've already done the alignment work for mRNA by STAR aligner …
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I am using the command
parsnp -g ../ref/AANRFQ010000001.1.gbk -d ./tmp_seq -p 8
but in spite of having everything in place, reference genome in ref/ folder, and all target .fasta files in ./t…