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Hi,
I just, want to know that it only works for genome sequencing, not for RNA sequencing. So are you planning to do an RNA sequencing simulator also included in this tool?
Thanks in advance
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Hi
I am trying to download and split files for SRR16784068.
In original file section, it says that this SRR file contains 6 fastq files (3 from RNA sequencing, 3 from BCR sequencing).
However…
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Thanks for making a such a beautiful program. I read the paper and it impute the genes in spatial data using scRNA-sequencing data. I was reading following tutorial
https://uniport.readthedocs.io/en…
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1. A comparison of automatic **cell identification** methods for single-cell RNA sequencing data
Article Link: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1795-z
2. Challen…
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Hi,
10x has a newish product ["flex"](https://www.10xgenomics.com/products/single-cell-gene-expression-flex) that allows for the sequencing of fixed nuclei.
It works based on [a set of 20 000 pr…
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It's OK In prod (e.g. here https://docs.lamin.ai/scrna#populate-metadata-registries-based-on-an-artifact)
I just saw it in a notebook from @sunnyosun
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So, I performed direct RNA sequencing on nanopore and got fast5 files for them. When I do tombo resquiggle, should me reference fasta file by DNA or RNA sequence in fasta?
For instance, if I sequen…
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Hi,
I have a dataset of NSCLC tumors that have both been whole-exome sequenced and RNA-sequenced. I have some more samples for which I only have DNA so I wanted to use ExTRECT to predict the T cell…
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Hi I'm trying to run import-rna with raw read counts. The results are very different from that by targeted DNA seq. The samples for RNA seq vs. DNA seq are 99% same so they are supposed to generate si…
laopp updated
5 years ago
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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?