-
To start with, I think there might be a confusion between enzymes that install methyl groups at adjacent nucleotides, and enzymes that install two methyl groups on the same N6-adenine. Only the latte…
-
ERROR ~ Error executing process > 'download_sortmerna:sortmernaGet'
Caused by:
Process requirement exceeds available CPUs -- req: 24; avail: 16
Command executed:
git clone https://github…
-
到Step 3. Removing rRNA Sequences with SortMeRNA
中Run SortMeRNA (~15min)
sortmerna \
--ref $sortmernaREF \
--reads results/2_trimmed_output/sample_trimmed.fq \
--aligned results/3_rRNA/aligned/sam…
-
- subClassOf OBI:0666666, RNA extraction
- has output (OBI:0002627, from #1036)
-
Hey, hello! I’m a bit confused about why I’m getting errors even though I’m using the same db file. Could you please help me take a look?
When constructing the index, I used the same db file, and bas…
-
Funannotate pipeline is awesome. I have two queries.
1) how to incorporate results of Barrnap (rRNA prediction) in final genebank and other annotated files.
2) Add tool to generate circos plot of…
-
Hi,
1. Can SO:0000325 rRNA_large_subunit_primary_transcript have the synonym "35S rRNA primary transcript"? It can be a narrow synonym if there are other large subunit primary transcripts of different…
-
RNAmmer is able to annotate rRNA genes containing gaps of Ns. Is this a feature that you would want to implement in Barrnap? See below for an example:
``` fasta
>rRNA_205522_53445-53560_DIR- /molecul…
-
The following error I received when I run the tutorial
(genovi) sam@DESKTOP-QP6LGUS:~$ genovi -i Paenibacillus.gbff -cs paradise --scale linear --alignment '
-
Hi Torsten,
I noticed Barrnap doesn't annotate well rRNA. My genomes have an intergenic spacer region of 150 bp in 23S rRNA and PROKKA annotates different CDS in that region. For that reason, no 23…