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Experiment: Single Cell RNAseq from 10x genomics
File Format: I have 2 Lanes (L001 and L002) and I also have for each of the lanes an R1 and R2 file
Reference Genome: Multiple HIV proviral sequences…
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2017.3.24 上午
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基因组
2017.3.24
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1.什么样的物种适合全基因组测序？
能被高引用的，才可能高分文章。
天山雪莲，从稀有、中药角度，老外估计不喜欢。
如果有天山雪莲的体外培养系统，做成耐寒的模式生物，则有可能发高分。
end9:45
2.技术流、思路哪个重要…
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I am interested to run your tool: Salmon. It is a great and easy tool to use.
I encounter this error by using:
```
./bin/salmon quant -t output.fa -g hgTables.gtf -l IU -a ../../pipeline/rna_seq/…
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Hi, I ran PASA with singularity to perform transcripts assembly and annotation update. The alignment assembly step ran successfully, but the update step ran with error, here are the error information:…
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Hi @rob-p @k3yavi
I am using salmon v1.0.0 and I am using the SAF method to quantify SE QuantSeq reads (Lexogen FWD) using these command line parameters
`salmon quant --threads 16 --noLengthC…
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Hi,
Thanks for providing such a useful tool.
I am running bambu on ferret sequence, and the gtf format as below:
```
NW_025422005.1 Gnomon transcript 20261931 20265242 . - . transcript_id "rna…
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Hi there,
This is a question not an issue. I was wondering if it possible that bambu could be used to ID operons in bacterial long-read cDNA sequencing. Conceptually, to me at least, an operon and ex…
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We're interested in quantifying isoform specific stability with a custom set of 3'UTRs
If we were to tweak the bam2bakR pipeline, supplementing a custom set of isoforms as separate ‘genes’ to a sta…
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Hi Eric,
After running minimap2, would it be necessary to collapse the redundant isoforms before using ESPRESSO?
Thanks~
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Dear VAST-TOOL Software Development Team，
Greetings! Thank you very much for developing such a great software, and now I have a small question for you: is it possible to handle long-read RNA-seq da…