-
Hi there,
I am trying to basecall pod5 files using guppy and it seems like it does not recognise the pod5 files. I am using version 6.1.7
Here is my script:
guppy_basecaller --input_path /mn…
-
It would be useful to have complete genomes in the repository for Selenobot purposes (necessary for extending predicted selenoproteins).
-
Hello, I would like to inquire about the input data format. When preparing the config.SFs file, I observed that it is possible to input non-chromosomal data. Consequently, I prepared two datasets.
Th…
-
## Describe the issue
Hi,
We are currently using the G2P plugin based on the the global MAF in the locally downloaded gnomAD variants (`gnomADg_r3.1.1_GRCh38` and `gnomADe_r2.1.1_GRCh38`).
The…
-
Hi, the software seems very good, but I am new in metagenome analysis and I have questions about the selection of appropriate reference genomes (indexes) when using 'hostile'.
1.
I read the "Refe…
-
### Research
- [X] I have searched the documentation and previously asked questions.
### Question about nf-flu
I've been successful in using NF-flu on flu A nd B sequencing data, but just got …
-
Hi,
I'm using this command to generate metagenomes from a set of genome fasta files:
iss generate --draft (list of *.fna files) --output --model nextseq --coverage uniform -p 128 -n 50M
Alt…
-
Hi Shujun,
I'm trying to wrap up some work on the Hesperis matronalis genome and am having a problem with panEDTA - it keeps timing out. I've cranked up the RAM to a terabyte and it still just runs…
-
Hi, I want to make sure that when I use smoove call for tumor/normal pairs.
Is there a requirement for the order of two bam files?
Which command is correct ?
The command is as follows:
`smoove ca…
-
Hello
I wanted to try out RawHash on some human genome data. I am still at the first step on generating the index.
For R9.4 I could create the index using the following command without any issue.
…