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Hello,
I'm trying to correct my reads with CANU.
I noticed that, when we increase the “genome size” the number of output reads also increases until it even exceeds the number of input reads.
…
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### Description of feature
Out of curiosity, I was running Camilo's small mixed dataset (mixed.csv), ran into an issue with the fungal sample (see issue 65), and I replaced the fusarium short read …
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Hi,
I have been trying to manually select the cutoffs by running "hist_plot.py". As I understand, we are supposed to select cutoff values from the count-read_depth image and manually change the "cuto…
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Hello there!
I have a question about the -M 2 option for identifying sample types using the barcodes.
I see that option 2 finds matched barcodes on both ends of sequence, and identifies pairs th…
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Hello,
Thanks for developing this tool! I'm looking to run it with nanopore long reads. Have you tried using it for that before? Could you share any experiences or insights regarding its application…
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Hi,
I'm running the nextflow pipeline and observed that I get 1,328,757 UMI after step 4a and 931,350 UMI after step 4b.
I figures that I get the same error as in https://github.com/ucagenomix…
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Hello, I'm getting a segfault when building the latest version: 817ba03fc19e0fb2076381eb9536ff99240bd468
Using this graphmap command line:
graphmap align --threads 1 --ref Chlamy_and_lambda.fa --…
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Please consider supporting the pairwise mapping format of minimap2:
https://github.com/lh3/miniasm/blob/master/PAF.md#paf-a-pairwise-mapping-format
Note that minimap2 also supports outputting SAM.…
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Hi,
I have used guppy 2.1.3 to perform basecalling of cDNA sequencing reads generated by nanopore sequencing. Basecalling generates multiple fastq files of the same library so I use cat to merge al…
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### Operating System
Other Linux (please specify below)
### Other Linux
Red Hat Enterprise Linux release 8.6
### Workflow Version
v.1.2.1
### Workflow Execution
Command line (Cluster)
### Othe…