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Hey folks, big fans of starcode. I really like this tool (well designed, Levenshtein distance, code is readable), and I'd like to use it for all my seq clustering needs. I hope y'all are continuing ac…
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External workflows, e.g. the once from the training material, should be updated regularly. Or even better should be a linked against the training material. Maybe we can pull them down before running t…
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Hi,
Maybe I am missing the option, but how can include a reverse primer binding site to make schematics of PCR amplicons (I can only find the "forward" Primer Binding Site?
Thanks!
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I was wondering if there's any plans to add "taxonomic dissimilarity" measures to `vegdist` (or if it is implemented and I missed it I apologize). [Clarke et al. 2006 ](https://doi.org/10.1016/j.jembe…
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Dear,
I find the ANCOM-BC approach to identify deferentially abundant taxa very interesting and was wondering if the estimated sample-specific offset term could be replaced by a known sampling frac…
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The `join` command currently just takes the union of positions and reads kept. While this is useful for joining non-overlapping amplicons, it will not treat primer binding sites correctly if the ampli…
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(args_oap) lishuxian@tong1:/mnt/md0/LSX$ ~/primerdesign/PMPrimer/pmprimer.py -f modified.fasta -p notsameseq -a muscle threshold:0.50 merge primer2 --debuglevel 1
序列集共有序列 144 条 / Number Of Sequece…
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Hi there,
Can I use trgt to call repeat expansions from short read data (illlumina data for example) ? If yes, is there any caveat?
Thanks in advance.
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It looks like nhmmer fails on phyloFlash v3.4.2.
I am running this command:
```
phyloFlash.pl -lib test_phyloflash -CPUs 32 -everything -read1 ../../data/interleave/JMF-2212-17-0001A.interleave.f…