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Dear @EMBL-PKU ,
I have multiple sites, but only one sample per site.
So my code is BASALT -a Y_assembly.fa -s Y.1.fastq,Y.fastq -t 64 -m 250
However, when I run to the third step, the following …
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Is it possible to use nanocomp to compar runs if each run contain several fastq.gz files ?
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I have Illumina novaseq, pair ended reads and I want to trim the non-internal adaptors from both ends. The adaptors I used for library praparation are NEB next adaptors for illumina. So I executed the…
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### Description of feature
UMI data is sometimes stored in a third FASTQ file, typically because the UMI is embedded in the index and bcl2fastq2 cannot combine it into forward/reverse. This produces …
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Hi @gbouras13,
Many thanks for your excellent tool. I am trying to implement Plassembler (v1.6.2) within one of my in-house Nextflow pipelines on a Linux machine. Plassembler analysis ran successfu…
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# Issue Report
Dorado read error correction: calling model for R9.4.1
## Please describe the issue:
Hi, I would like to use the `dorado correct` function to correct R9.4.1 data, but it is unclear…
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I was double checking the bioconda version of Nonpareil 3.5.3, but unfortunately everything I try results in the following error:
```
$ nonpareil -s test.fastq -T kmer -f fastq -R 10 -v 10
Nonpar…
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Hello,
I am opening an issue as I am trying to install and run ARGprofiler on my laptop for the first time, but i run into an error.
- My machine runs Ubuntu 22.04.4 LTS and I use mamba for instal…
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Inquiry regarding demultiplexing of FAST5/pod5 files using Dorado
Hi there,
I recently conducted direct cDNA sequencing using a barcoded library, which resulted in the generation of FAST5 files (l…
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Hi,
I was using debar on my fastq file with the input and output (default) as fastq.
Later, I wanted to process the file further with usearch (dereplication) but it says
`---Fatal error---
Bad FA…