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Hi,
I have two questions :
1. How can we use single cell data normalized with SCT ? I have the following error :
Error in as.matrix(sc_dataset@assays$RNA@data) : no slot of name "data" for …
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cas : https://app.datasubvention.beta.gouv.fr/search/W011000016
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### Description of feature
The current rna-seq pipeline, generates two bigwig files one each for each strand. it would be great if like chipseq pipeline, it generate only one bigwig files, would be h…
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Hello!
I now have RNA-seq data and full-length transcriptome data from several tissues, and I used "Trinity genome-guieded" to get the transcript "Trinity-GG.fasta" of the RNA-seq . At the same time,…
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I have been using TEcount for estimating TEs in total RNA-seq data which has worked great. However, I want to combine this with methylation data and there we have methylation differences at the transc…
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### Is your feature related to a problem?
In one of my samples, wf-single-cell fails to correctly find the cutoff in the knee plot and returns an excessive large number of cells. Consequently, when l…
ddiez updated
18 hours ago
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### Description
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### (Required) What is this issue most closely r…
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Thank you for creating the snoGlobe tool. It has made easier to find the potential interactions between snoRNA and target RNA. I am currently using a DNA fasta file from Ensembl to identify possible m…
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Can't locate Env.pm in @INC (@INC contains: /home/groups/carilee/rna-pipeline/RSEM /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/pe…
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Here is my code
```R
cds_raw