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Hello,
I am running a very large assembly on a supercomputer that has a local disk to page off of. Is there any way I can tell megahit to stop after a certain kmer point (or if I told it only to …
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I have 106 human gut samples (average 22,078,021 paired-end reads 2x150bp) and want to use megahit for assembly, but I don't have enough computer resources (64 threads + 126G RMD). I want to assemble …
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hello,
I used metaspades.py and got an err code: -6 after K77. Could please help with the issue ?
Here is the spades.log.
Thanks you !
[spades.log](https://github.com/ablab/spades/files/35794…
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Hi, I'm trying to submit an assembly of ERR2114807 and am receiving a validation error;
> 2019-07-29T11:59:10 ERROR: Flatfile contains non-ascii characters: "leaf juice of 5g whole plantlets, brief…
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Dear, I have metagenome assembly of two samples that I am trying to bin. Although I have a high abundance of Proteobacterial reads, after binning I don't get any Proteobacterial bin. What could be the…
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Dear developers,
In the manual, metaspades.py only supports a single paired-end library for metagenome.
I use spades.py for assembling multi-pair-end libraries for metagenome, but it finished wi…
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Hi,
firstly, thanks for introducing such great tool for identifying snps in isolates.
I am trying to use the metagenomes-assembled genome (>300 coverage) to map back to the original reads, but th…
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Hi Mike,
I was wondering if you would have any ideas on how to conveniently mass-generate a mapping file (genome_to_id_map.tsv) for using the -m flag on GToTree (as I don't want to sit and manuall…
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I put the files on eagle under the emma folder. Here is what you have:
orf.fna - entire metagnome assembly
proteins.prodigal.faa - translation of metagenome assembly
orfMG...fna - assemblies of ind…
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Hi, thanks for this tool! Very easy to use and great documentation. Still, I'm struggeling with the output of my run. I'm working on a set of environmental samples that I would like to use for compara…