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- Focus: Design a project to show the limitations of RNA-Seq and the effectiveness of single-cell analysis in gene expression studies
- Key Components: RNA-Seq data analysis, single-cell sequencing t…
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I have run the following command for RNA seq data and the output vcf size is very less and important variants are missing
BIN_VERSION="1.5.0"
```dockerfile
docker run \
-v "$(pwd):$(pwd)" \
-w…
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Hello,
I have got the following error while running show.velocity.on.embedding.cor function.
fit.quantile
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Hello,
I am running a single-cell analysis. I want to compare three conditions: A, B, C. These conditions seem to be the main differentiating parameter as the UMAP shows three clearly separated isl…
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Hi,
I am using metatranscriptome data to identify bacteriophages using the vs2 tool. The problem is that I am unable to understand how to know, which virus is identified from the viral contigs. Al…
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Hi there, if i wanna use 4sU pulsed total RNA to construct strand-specific library followed by the SLAM-seq protocol with the exception of library construction step, in this context, rRNA will be remo…
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### Description of feature
My question is which assay type (GRO-seq, PRO-seq .. etc.,) should I use for EU-seq analysis? This is the outline of EU-seq (PMID: 34485932). Briefly, nascent RNA is labele…
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This is a meta-issue recording action items from a review of biomedical articles that cite SciPy and the results of a survey sent to authors of over 50 of these papers. In some cases, we can offer to …
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To reduce the footprint of larger workflows it's very useful if temporary files (which are marked as such) could be **automatically** deleted once they are not used anymore. Yes, this breaks reruns, b…
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I used stringtie to analyse the output of my hisat alignment in >30 human samples in parallel and detected single samples with FPKM values of 0 for several genes although the regions are well covered …