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Greetings,
I'm trying to demux a pod5 with skipped reads. I merged the pod5 and basecalled it using dorado and demux'd it the bam into fastq files. I convert the merged pod5 with blue-crab into a …
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I converted fast5s to a ~900 GB single pod5 file (v0.2). When basecalling, it is taking up a ton of memory:
This doesn't happen when I call from fast5s. Is this normal?
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Dear Dorado developers,
I am replicating a Dorado pipeline from a sequencing center to obtain the same results in **fastq** format. However, the sequencing center mentioned that they use the cloud …
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### Ask away!
Hello, I am encountering the same issue. I am attempting to use dorado through epi2me and docker. Other epi2me workflows work for me as does base calling through guppy using cuda from t…
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### Assembly Region
chr5_PATERNAL:47422349-47422811
### Assembly Version
v0.7
### hifi_evidence subregions
47422349-47422811 (Low)
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### What happened?
Hi all,
I am executing EPI2ME in windows 11 and I have problems when wf:dorado is runing
Can anyone help me?
Thanks in advance
### Operating System
Windows 11
### Workflow …
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Hi all, setting myself up here for a potentially dumb question. We recently upgraded MinKNOW to v4 and I am getting inconsistent results with readfish. I'll start with the basics.
Running 4.0.11 a…
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`dorado` seems to use all available CPU resources, spawns a lot of threads (corresponding to the number of CPUs, including a few `cuda-EvtHandlr` threads).
Will there be a parameter for restricting…
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Running dorado 0.1.1 with any model on a fresh install of ubuntu 22.04 gives the following error. However, the base calling still appears to be working despite this error. Any fix?
`[2022-12-30 09:46…
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We have recently been trying out running Dorado as part of an automated pipeline to do methylation calling using the all cytosine model from some recent Promethion runs. We have noticed that on our c…