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Hi,
I was trying to build the spliceu index for the ensembl rat genome.
I used conda to install simpleaf
`mamba create -n af -y -c bioconda -c conda-forge simpleaf piscem`
Then I downloaded unzi…
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- [ ] ned1 as per PMID:26774782
The second
annotated 150bp-long intron is retained, resulting in insertion of additional 50
amino acids in a region between the N-lipin and HAD-like domains
old
…
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Hi all,
Thank you very much for developing these helpful packages!
At the 'isoseq refine' step I set the 'require-polyA' parameter thus I guess the refined molecules should have polyA tails. Howev…
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### Project short name:
SkinSystemicSclerosis
### Primary Wrangler:
Ida
### Secondary Wrangler:
Ami
### Associated files
* Google Drive: [folder](https://drive.google.com/drive/folders/1tkFLKu…
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Hello!
Thank you for developing this great tool! I tried using it, but unfortunately I got the following error:
Traceback (most recent call last):
File "/home/i/ikaufhol/software/TEspeX/TEspeX.…
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# 生信爱好者周刊（第 137 期）：
这里记录每周值得分享的生信相关内容，周日发布。
本杂志开源（GitHub: [openbiox/weekly](https://github.com/openbiox/weekly)），欢迎提交 issue，投稿或推荐生信相关内容。
[「生信周刊讨论区」](https://github.com/openbiox/weekly/discuss…
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HI, Does Kraken work properly on RNA-seq data? Does Kraken attempt to map kmers in both directions (regular and reverse complement)? Since RNA reads might come from either strand of the reference.
Th…
gevro updated
4 months ago
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你好，我在构建人的带荧光参考基因组的时候遇到了个问题，我使用的是最新的Gencode的fasta和gtf文件，在run的时候发现添加的一个荧光基因会被跳过，rna run输出的信息如下：
2024-06-27 17:13:42,165 - data - INFO - Executing command: /home/dell/anaconda3/envs/dnbc4tools/lib/pytho…
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I'm pretty sure all the executable paths are updated now for my configuration. Everything runs fine until I get to line 35 in 1_kallisto.smk (rule kb_velocity). I am getting the error below and am fin…
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# Issue Report
## Please describe the issue:
Hi,
I have been re-basecalling some Nanopore cDNA reads generated a couple of years ago (originally basecalled with Guppy 6.1.5 high-accuracy). Doi…