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### Description of the bug
In the current `dev` branch the modules `MOSDEPTH_AMPLICON` and `PLOT_MOSDEPTH_REGIONS_AMPLICON` seem to be always skipped.
This may be related to [these changes](https…
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I am getting the following error when running the get-ncbi-data
This occurs even if I rearrange the order of parameters and outputs, and by changing the query to be enclosed with "" instead of ''. T…
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Hello!
Thank you for the tool. I run it with some samples, and the results are promising.
Currently, I am testing this tool for one pipeline that should handle many ONT barcoded samples.
I have alr…
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Hi,
I found a typo in the output VCF that is causing errors when parsing. The AF parameter has NUMBER=1, so it must have exactly 1 entry, but with the recent update of adding af for all alternate all…
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### Description of the bug
Hello,
I am trying to run this pipeline to demultiplex an illimina miseq dataset of ITS amplicons.
I am kind of confused what should be in the samplesheet. In the `.nex…
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I have a data from 16S/18S/ITS Amplicon Sequencing. Can I use dada2 for analyzing such data? Should I divide them into 16S, 18S and ITS subsets as the reads have different lengths?
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### Ask away!
Dear developer,
We are trying to use your wf-amplicon workflow on Epi2ME platform to quantify variants in amplicon libraries generated from the bacteria Desulfovibrio vulgaris. We ar…
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Dear Clair3 dev team,
## Background
I am using Clair3 for SNV/indel detection from ONT sequence data. The sequence data is from an amplicon sequencing experiment (high read coverage) of a mixed SA…
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When I try to compile using OpenMP instead of mpic++, I am getting an error from make:
````
gcc -O3 -Wall -fopenmp -std=c++14 -DUSE_BLAST_DB -I. -I/mnt/Storage/genomics/tools/ncbi-blast-2.12.0+-s…
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I am interested in duplex calling using Dorado but have a few questions that I can't find the answers to.
The Dorado readme describes a process to split pod5 files to enable distributed duplex call…