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I'm struggling with FAST5 playback as described in the README with MinKNOW 20.06.5. I get zero pores and no reads (like #65). However, I'm trying to replay the bulk fast5 linked in the README.
Of …
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When basecalling modified bases with `dorado` v0.5.0, using the modification models `5mC_5hmC` and `5mCG_5hmCG` stall basecalling randomly, i.e. sometimes `dorado` finishes basecalling, but mostly it …
janpb updated
9 months ago
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Hi Hiruna,
This looks like a really great tool, and I wanted to thank you for your detailed documentation of distinction between generating nucleotide-anchored signal with f5c and what you get from…
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# Issue Report
## Please describe the issue:
I am trying to run dorado on a .pod5 file. However, when I do so, I get an error:
[2024-04-19 23:00:41.196] [error] finalise() not called on a Hts…
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Hi dorado Team,
I have some issues with old data converted to pod5. I had a lot of data set which I currently coverting to pod5 for re-basecalling with dorado. However, for some of the sample, althou…
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Type: Bug
# Behaviour
Pylance keeps crashing since yesterday, it has been working fine before. VSCode is up-to-date, I also switched to the pre-release version of Pylance, reloaded the window …
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Hi,
We've been using Flye to assemble Atlantic salmon from ONT long reads, but experience that it requires a lot of time.
The genome is comparable in size to human, ~2.9Gb, but is additionally dupli…
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# Issue Report
## Please describe the issue:
I ran `dorado basecaller sup --kit-name $kit_name --modified-bases-models dna_r10.4.1_e8.2_400bps_sup@v4.3.0_6mA@v2,res_dna_r10.4.1_e8.2_400bps_sup@v…
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Dear Authors,
I run dorado with bacterial methylation model from the rerio, res_dna_r10.4.1_e8.2_400bps_sup@2023-09-22_bacterial-methylation
My command is:
dorado basecaller dna_r10.4.1_e8.2_400b…
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Hi, Thanks for this great tool. I have a question regarding the computation of the mean read quality score in the NanoStat.txt file: why is this value different when computed from the basecalling bam …