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Hello,
I try to run Pilon with the following command:
```
java -Xmx512G -jar pilon-1.23.jar --genome genome.fa --frags illumina_alignment.bam --nanopore nanopore_alignment.bam --changes --vcf --t…
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Do you recommend any QC on the short and / or long reads before submitting to OPERA-MS for the hybrid assembly of metagenomic samples?
For short reads that would include removing adapters, trimming…
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Hello,
I installed both version of Scallop-LR v0.9.2 and Scallop v0.10.4 and tried to run both on Nanopore data. Scallop-LR is not working (it runs but does not return anything) but Scallop is wor…
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R N A F L O W : R N A - S E Q A S S E M B L Y & D I F F E R E N T I A L G E N E E X P R E S S I O N A N A L Y S I S
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Hi,
Quick question: what does it imply to use the `-L` option ? Long reads have multiple differences between datasets so I would like to know how to adapt this option. More precisely, could we repro…
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Hi,
I'm currently doing 6mA methylation detection with megalodon. But there is a issue that I cannot figure out.
The error file for batch running always showed "Segmentation fault".
I also che…
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I am getting this error while trying to upload my fast files with the Webin uploader application. The upload works fine but a few days after I get this error below by email for some files. Some FASTQ …
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Hi
Any plans for expanding to include models for 6mA methylations?
Best regards
Rasmus
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Hi,
I am running CONSENT to correct some Nanopore reads and I encountered some errors.
First, I tried running directly in the command line in Linux with 4 cores and 4 GB each with the following …
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Hi,
Recently, I am doing some analysis of Nanopore cDNA PCR data. When I processed NGS data, I used Picard to remove duplicates, so I also used picard to process ONT cDNA, and it turned out that mos…