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(i.e. combine the way demultiplexed seurat object is constructed)
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Hi,
If I run lotus2 with `-saveDemultiplex 3` it results in an error. Can't say exactly what is happening.
I don't find the log file where the error should be described?
The lotus2 version I us…
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**Is your feature request related to a problem? Please describe.**
Projects with many samples can produce a large amount of data in one measurement.
To analyse effects only observed in a subset of…
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Running command line: `python guideseq.py all -m manifest_1.yaml`
after demultiplexing begins I receive: `IOError: [Errno 24] Too many open files: ... `
Upon inspection of the demultiplexed output…
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### Describe the feature request
Is there a way to implement SNI-based routing, that is to demultiplex a single connection onto different targets?
This is how I currently implement it with [Caddy]…
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Hi ProteoWizard team,
I am using MSConvert to demultiplex DIA file acquired using 8m/z staggered window on Orbitrap 480 Exploris. I used 8 m/z staggered window setting recommended in Pino L et al.,…
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### Description of feature
Future plans for demultiplex workflow:
Attempt to convert into atomic subworkflows:
1. wrap different demux strategies into subworkflows:
- demultiplex_illumina (`bcl-…
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Hi Dorado team,
I have performed dorado duplex calls, then from the resulting BAM file, I have used the below command to only compile a bam file consisting of duplex calls:
samtools view recovered…
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### Describe the bug?
Multiplex - Many to 1 Data Transmission
Demultiplex - 1 to Many Data Transmission
When using Value Multiplex it is a 1 to many operation and when using Value Demultiplex i…
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Hi,
`sc_demultiplex_and_count` is running till the end without bugging but then the `count` folder is empty (no .csv written). It is quite frustrating, as there is no error so no idea why it is not…