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Describe the issue:
We are currently using two different long-read sequencing systems: PacBio Direct and PacBio Capture.
We are encountering two specific issues:
1. **PacBio Direct Data (top t…
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Preferred Term: long-read RNA sequencing assay
Synonyms: long-read RNA-seq
Definition: An assay that determines the sequence of an RNA molecule using single-molecule technologies to allow longer rea…
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Adding a new stage and jobs for a pipeline to be used by the rare-disease team.
The stage will create CRAM files from BAMs, not by re-aligning the BAMs, but by converting them.
### How this stag…
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Hello,
I'm analysing single-cell full-length RNA sequencing data. I would like to perform an analysis on alternative splicing exons, which has been mentioned multiple times in your research group's…
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Hello! Thanks for your helpful tool!
I am trying to assemble long-read sequencing data with short-read polishing. My long read data (nanopore direct RNA sequencing) are from the animals under 25 de…
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conda is leveraged for installing (some) dependencies (at least, the recommended install method), but conda is not fully leveraged via a bioconda recipe for installing scNanoGPS.
Are there plans on…
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Hi this might be off topic but have you tried using long read data on AA?
I'm planning to generate HIFI reads for some of my samples and wonder if it's any good with AA?
Probably not as HIFI won't …
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Hi Andre,
Thanks for your great contribution to the Bambu pipeline. I am running it to identify novel transcripts from long-read sequencing data.
However, I found multiple entries with the same ex…
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Dear bcbio developers,
For some of the species that we work on we are now starting to receive significant numbers of long read sequenced samples.
The samples are sequenced using nanopore, PacBio …
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Hello! Thank you for providing such a useful software.
My original hic data are all paired reads of equal length of 150bp. I hope that the simulated data are also regular 150bp paired reads.
Bu…