-
Hi. Thanks for QUILT - it is really great!
I was wondering if you have information on the effect of data quality (phred scores) on the results. Basically I am wanting to work out if trimming the da…
-
Hi, I tried to visualize the provided hand pose, the quality of MANO Sequence is in very low quality an can barely be used. Here is some visualizations I managed to get. Can you check and let me know …
-
Hi,
I tried running lotus2 but got the following error:
This is sdm (simple demultiplexer) 2.17 beta.
Checking for switched pairs.
Run with 12 cores.At lotus2/SRR13436190_1.fastq:
Undecided f…
-
Hi rMATS team,
I would like to apply rMATS on our recent RNA-seq data. During QC check of the fastq files using FastQC, I see a high level of sequence duplication (please see the attached fig). Do …
-
Hi,
I was wondering if there are plans to update winnowmap to include sequence information even if mapping quality is 0 in BAM file? We noticed that when mapping quality is 0 for a read, winnowmap …
-
Update [fastqc_per_base_sequence_quality_dropoff.py](https://github.com/aomlomics/tourmaline/blob/develop/scripts/fastqc_per_base_sequence_quality_dropoff.py) to work with fastq_summary.qzv output
-
To make it clearer that sequence-quality start/end refers to sequence location, might be cleaner to move sequence-quality under sequence as a child.
-
Hi, I realised that the lower half of the sequences in *structure.gz file are not in order.
In fact, it's pretty clear that those sequences are low quality because IMonitor can't classify its v-genes…
-
Hi,
I am trying to get the matrix file from paired single cell rna seq files that i obtained from singleron.
However, I encountered this error, and am unsure how to proceed.
This is the log.out …
-
I am using nextDenovo to assamble genome with ONT reads.
The long reads usually have low base quality.
And I wonder how these sequences will be handled in Nextdenove? Discard or correct?
![image]…