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kalekundert
/
stepwise_mol_bio
MIT License
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digest: use recommended reaction volume
#44
kalekundert
opened
1 year ago
0
digest: make enzyme mix to avoid pipeting small enzyme volumes
#43
kalekundert
opened
1 year ago
0
digest: option for −enzyme controls
#42
kalekundert
opened
1 year ago
1
ligate: confusing error message if MW can't be found
#41
kalekundert
opened
2 years ago
0
ligate: warn when using phosphorylated backbone
#40
kalekundert
opened
2 years ago
0
digest: add phosphatase option
#39
kalekundert
opened
2 years ago
2
ligate: option to *not* merge reactions
#38
kalekundert
opened
2 years ago
0
direct/serial: calculate initial dilutions
#37
kalekundert
opened
2 years ago
0
digest: directly specify volume of DNA
#36
kalekundert
opened
2 years ago
0
Add full plasmid sequencing protocol
#35
kalekundert
opened
2 years ago
1
Add electrotransformation protocol
#34
kalekundert
opened
2 years ago
0
Add "unbiased" PCR protocol
#33
kalekundert
opened
2 years ago
0
Think about how samples load the FreezerBox database
#32
kalekundert
opened
2 years ago
0
thermocycler: allow steps to be named
#31
kalekundert
opened
2 years ago
0
sequence: add way to specify colony PCR primers
#30
kalekundert
opened
2 years ago
0
spin cleanup: warn if column capacity is exceeded
#28
kalekundert
opened
2 years ago
0
pcr: allow annealing temperature to be a range
#27
kalekundert
opened
2 years ago
0
Add an E-gel protocol
#26
kalekundert
opened
2 years ago
0
Migrate to BYOC
#25
kalekundert
closed
2 years ago
1
Refactor unit tests
#24
kalekundert
closed
2 years ago
1
ethanol: read presets from a config file
#23
kalekundert
opened
2 years ago
0
Add a trizol protocol
#22
kalekundert
closed
2 years ago
1
pcr: allow multiple products to be specified
#21
kalekundert
opened
2 years ago
0
pcr: add an option to explain how Ta was determined
#20
kalekundert
opened
2 years ago
0
Add a plate-pouring protocol
#19
kalekundert
opened
2 years ago
0
digest: unnecessary denature step
#18
kalekundert
closed
2 years ago
2
Universal config options
#17
kalekundert
opened
2 years ago
0
Add an overlap extension PCR protocol
#16
kalekundert
opened
2 years ago
1
pcr: add touchdown options
#15
kalekundert
opened
2 years ago
0
pcr: require extension time
#14
kalekundert
opened
3 years ago
0
Add a gel purification protocol
#13
kalekundert
opened
3 years ago
2
Add a protocol for making buffers
#12
kalekundert
opened
3 years ago
1
Set command-line defaults from FreezerBox database
#11
kalekundert
closed
2 years ago
1
Add a "miniprep" protocol
#10
kalekundert
opened
3 years ago
0
gel: add pre-running options
#9
kalekundert
closed
3 years ago
0
Add a "transformation" protocol
#8
kalekundert
closed
2 years ago
0
ethanol: add some line breaks
#7
kalekundert
opened
3 years ago
0
Add a generic thermocycler protocol.
#6
kalekundert
closed
2 years ago
1
Be more clever about caching reactions
#5
kalekundert
closed
3 years ago
1
digest: Add option to skip heat denaturation step
#4
kalekundert
opened
3 years ago
0
ivt: Incorporate NEB recommendations for purifying the template
#3
kalekundert
closed
3 years ago
1
ivt: Allow the aliquot concentration and volume to be specified
#2
kalekundert
closed
3 years ago
1
ivt: don't allow negative solvent volumes
#1
kalekundert
closed
3 years ago
1