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Hi all,
I've been trying to realign some CEL-SeQ2 data: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE157504 The original alignment/counting was performed using zUMIs.
The UMI+BC…
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Hello,
I followed kallisto_bustools commands in guide you provided.
Here is my code
`kallisto bus -i $work_p/homo_ercc.idx -o nn84/ -x 0,6,14:0,0,6:1,0,0 -t 4 $seq_p/RPI2_S1_R1.fastq.gz $seq_p/RPI2…
YOU-k updated
4 years ago
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Project Title: Systematic comparative analysis of single cell RNA-sequencing methodsProject Short Name: scRNAseqSystemicComparisonProject UUID: 88ec040b-8705-4f77-8f41-f81e57632f7dWrangler/s: Chris,,V…
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**Goal**
Development of a stand-alone fastq validation tool.
A flexible tool that can be applied to different single-cell technology types to check for the expected cell barcode.
It should also r…
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Project Title: Systematic comparative analysis of single cell RNA-sequencing methodsProject UUID: 88ec040b-8705-4f77-8f41-f81e57632f7dWrangler/s: Chris,,Villarreal; William,G,Sullivan; Parisa,,Nejad; …
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Hi,
It is unclear here and in your paper what database you are using at the time you are aligning reads against the reference with bowtie2.
Is the following what you have done ?
1. Reads were a…
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Hello,
First off, thanks for putting together an awesome tool. I'm a new user to rliger and was wondering if you had any guidance on how to handle integrating datasets from different analysis platf…
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Dear developers,
Congratulations and thank you for developing such excellent tool.
I was wondering that would Augur still works if I have two conditions sequenced by different protocols? For example…
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When trying to run Singler 0.2.2 with Seurat 3.0.0, I get the following error:
```
singler = CreateSinglerSeuratObject(counts=input2,
annot = NULL,
project.name="hihi",
min.genes = 500,
…
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# Design (@brianraymor)
**In Progress**
## obs (Cell Metadata)
...
### genetic_ancestry_ontology_term_id
Key
genetic_ancestry_ontology_term_id
…