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### Description of bug
We performed an hybrid assembly using metaspades (ONT + SR), we had really huge coverage. Nonetheless, one of the final we discovered that there were 5 minority SNPs that were …
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Hey Rhys
I was wondering why the same long reads used for the metaflye assembly are being used for the spades hybrid assembly with Short reads that did not map to the long read assembly. Wouldn't …
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There are two hybrid assemblies that I would like to add to YEAT's arsenal: 1) Unicycler, and 2) Trycycler.
As of right now, YEAT is able to use Unicycler with paired-end read data. In the future, …
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Hi,
We have 50x Illumina paired-end and 7x ONT reads. Is QuickMerge able to produce a hybrid assembly out of these data?
Thank you in advance.
Michal
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### Description of bug
I was running Unicycler to assemble a hybrid genome and encountered the following error related to SPAdes:
WARNING: Try to use logger before create one. Level=ERROR. Messa…
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Dear Developer,
I am using SPADES 3.10 for 50X illumina and 10X pacbio assembly. The genome size is 6G and my RAM is 1T.
My command line is:
spades.py --careful -t 24 -m 980 --dataset dataset.yaml…
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Many at ATCC were interested in using a combination of Illumina and PacBio to do hybrid assembly.
If we can do that using SPADES it is not well documented.
If we can't do that, we should consider ad…
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I want to ask if I have 20X illumina corrected pacbio reads, Should I use falcon for overlap graph construction? Because https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/Large-Genome…
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I am assembling a Drosophila genome with Illumina paired end , Illumina mate pair reads along with nanopore long reads. Size of the genome is ~190 MB.
Find the necessary files here
[params.txt](http…
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Is it a good idea to use Ococo as a long-read assembly polishing tool?
I.e. assemble w/ e.g. Canu, and then map Illumina reads to that assembly, having Ococo compute the consensus.
Thanks