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Two possible additions
### An equivalent to the `-m` option in ska1:
> Finally, the base call for the middle base in the split kmer is filtered to remove any bases where the minor alleles are foun…
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Have successfully installed all the software and then run
`$ ~/bin/doctor.py`
Below is the output
`$ ~/bin/doctor.py
# Doctor! Doctor! Give me the news.
# Checking symptoms ...
# bwa …
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Vsearch supports --fastq_join in which "sequences are not merged as with the fastq_mergepairs command, but simply joined with a gap."
**Addition Description**
It would work like the existing [me…
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Hello,
I am relatively new to Cutadapt and therefore this question might have been asked before or it may seem stupid.
I am using Cutadapt to trim the primer sequences from several metabarcoding…
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**EDIT I guess it would help if I were to read the publication to see especially the figures on time and amplitude domains' effects on base identity.**
Hi, thank you very much for developing this t…
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Hello, not really an issue, just some information regarding FastQ/Fasta parsing
FastQ parsing in Nim is implemented here https://github.com/andreas-wilm/nimreadfq, which uses Heng Li's superfast ks…
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I understand `splitcode` has many usages, and some of them involves substituting one sequence with another, such as `--assign` or `sub`, will, in a manner of speaking, make the corresponding quality s…
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Hi, Thanks for a quick tool.
I've been using this to UMICollapse my bam files.
Now I want to utilize it on fastq files.
I am confused by the statement:
"fastq: the input is a FASTQ file. This…
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I'm running fastq-sample from the current HEAD like this:
`./src/fastq-sample -n 10000 -o downsampled -s 12345
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I'm trying to replicate read pair assembly that I've tested with `usearch` and want to replicate with `vsearch`. In the [`usearch`](https://www.drive5.com/usearch/manual/merge_options.html) documentat…