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Dear developers,
would you be able to provide an RNA model for bonito somewhere
bonito basecaller **rna**_r9.4.1 /data/reads > basecalls.fasta
Thank you
Christoph
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Is it possible to use the tool for evaluating correction of the cDNA reads obtained for transcriptome sequencing? Would it be enough to substitute reference genome with the reference transcriptome?
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Hi Darcy,
just tried running panTE over a few new assemblies and keep running into following error:
```
Error executing process > 'runInfernal (ArTR9571.contigs.racon.medaka.mito)'
Caused by:
…
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The manual of Hifiasm states that Hifiasm can use ultra-long Nanopore reads. Can ordinary Nanopore reads be used by Hifiasm? Since ordinary Nanopore reads are usually longer than HiFi reads, it seems …
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I'm using `whatshap phase` to phase variants called from Nanopore sequencing data. I feel confused by some of the phasing results as the split reads do not appear to be consistent with the phased SNPs…
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A pileup of uncorrected Guppy v3.6 reads produces a better guess at the true sequence compared to corrected reads. The errors are in deletion variants and only in specific locations; for the most part…
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Hi,
Whenever i run a nanopolish polya run with my dRNA dataset i get an empty file with each read saying 'NO FILE LOADED'. Commands and output as below:
Command: nanopolish index -d /20230927_104…
glf20 updated
10 months ago
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Dear Unicycler team,
I chose Unicycler to assemble _de novo_ bacterial genomes with ONT sequences, because I tried with several ones and Unicycler gived me better results.
However I start to obser…
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Hi Ben,
I have been using DADA2 on illumina sequenced COI data for the last year and it has become the backbone of my bioinformatics pipeline. We now have a PhD student in our group developing meta…
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I'm running nucdiff on a uge cluster, attempting to use 10 processor. The process starts and appears to go well but I never get any output. I'm including my command line and the output.
If you can …