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Hi,
So, I used TGS-GapCloser to improve a 800 Mbp chromosome from a 10 Gb genome assembly, using 84 Gb of PacBio long-read data (9x).
Also I included the following arguments in the tgs command:
…
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Hello!
In my current project, we used DADA2 on a set of 16S Full-length PacBio CCS reads including the Zymo Mock sample.
Read tracking showed that I lost ~50% of the reads after the filtering step…
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Hello,
I am trying to use Flye to assemble a plant genome and I am having the following problem, could you please help me?
My script is the following:
#!/bin/bash
#PBS -N flye_completo
#PBS -l …
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Hi~
I want to use IsoQuant to quantify the long RNA data of PacBio CCS.
And my code is "isoquant.py --reference /data/Wangmeizhen/software/Cogent-master/Amana/WMZ1424/collected/WMZ1424_clustered.h…
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Hello,
I am using FMLRC2 using de bruin graph from RNA-seq to correct PacBio Iso-Seq data to see their impact.
I found small fraction of PacBio Iso-seq reads are shortened >500bp.
Could I turn of…
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**I have read the paper (https://doi.org/10.1038/s41467-020-15171-6)
and the manual (https://flair.readthedocs.io/en/latest/) and I still have a question about**
- something else
Hi Thanks for …
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@benjjneb
Hi,
I used to manipulate MiSeq reads on Qiime2 where I can use DADA2 plugins along with other q2-plugins easily. I need to export the sequence data as fasta file collapsed at specific t…
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Hello,
Thank you very much for providing the software. However, when I run deepvariant v1.1.0, I encounter the following file error. I did not encounter this error before. I hope you can help me so…
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Hi, I used hapo-g for a while now, and recently just found that somehow for two of my assemblies, hapo-g add `=` in the genome sequence.
I didn't notice it until I used the annotation lift-over to…
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To Whom It May Concern,
When trying to quantify isoform expression for a dataset (attached) by "**python $LIQA_PATH/LIQA.py -task quantify -refgene $refgene_PATH/chr1.refgene -bam $data_PATH/test1.…