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Hi @alexomics,
I have same RUN ERROR issue on my demo run with in minutes of loading MinION data using playback option in MinKnow UI. I have a MinKnow with Dorado and running in a UBUNTU 22
Not …
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Minimap2 is probably the best alignment algorithm when aligning nanopore sequencing data, and samtools lets you work with those alignments to produce useful output information. In fact, 3 of the parse…
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### Operating System
CentOS 7
### Other Linux
_No response_
### Workflow Version
v1.8.3
### Workflow Execution
Command line
### EPI2ME Version
_No response_
### CLI comma…
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Hello,
Happy new year and I hope you are well!
I was trying to run MetONTiime on my university's HPC with Singularity, on 44 samples (using SILVA 138 database for 16S rRNA). Everything ran well …
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Hello!
Could you please provide me with the information on your plan about adapter trimming incorporating to the Dorado. It is the feature I rely on in my workflow and it was that I used in guppy. No…
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### Operating System
Linux
### Other Linux
_No response_
### Workflow Version
v0.3.0
### Workflow Execution
EPI2ME Desktop application
### EPI2ME Version
_No response_
##…
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Others issues have explored this but nothing addressed my issue of performance/speed. I'm trying to follow these suggestions:
https://github.com/nanoporetech/dorado?tab=readme-ov-file#improving-the-s…
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### What happened?
Hello,
I have used oxford nanopore's direct cDNA sequencing kit to obtain transcript information from 18 different samples. Right now I am trying to run a differential expression …
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Hi author,
Thanks for this very useful tool!
I had this problem several times and also tried to solve it, but failed. Here is the issue:
Traceback (most recent call last):
File "./scNanoGP…
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### Ask away!
Hi,
I am running the test on the Debian HPC cluster with singularity configuration. The workflow stops at mod:getVersions due to "Python not found" although I do. Not sure what mig…