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mRNA methylation is now gone, but it seems odd that we have the other methylation terms.
@pgaudet Should this branch have "do not annotate" added, pending obsoletion?
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Hi,
I have a set of full length 16S rRNA genes (~500) that I want to map against other studies with the goal of determining the occurrence (maybe even relative abundance) of my 16S in other microbiom…
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### Description of feature
Here I attempt to break down the required/desired modules for each processing stage of the workflow:
### Pre-Processing
- [X] Quality Control (FastQC)
---
- [X] Cu…
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## Initial impressions on MAGinator
### Parameters:
**Downsample size**: 5000
**Refined set size**: 100
### Initial MSE improvement
![image](https://user-images.githubusercontent.com/4901…
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Hi Alex,
I am currently working on a RIP-seq experiment and using STAR to align my data. I noticed that for the wild-type (WT) sample, about 20% of the reads are multi-mapped, while in the mutant s…
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Hi there!
I utilized EMU to classify 16S and 18S rRNA amplicons sequenced using nanopore sequencing with the SQK-NBD114.96 kit. However, I noticed that some of the taxonomic assignments made by EMU …
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I have a `SOMA` object that I created from AnnData. It seems (?) to be valid:
```R
> soma$obs$to_dataframe() |> rownames() |> head()
Reading AnnotationDataframe into memory from 'file:///vast/scr…
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## Bug Report
CollectRnaSeqMetrics failed to work for the RIBOSOMAL INTERVALS on the 639th sequence of reference genome, but is OK for the INTERVALS on the 25th/1st sequence.
### Affected tool(s…
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Hi, developer
I used fastp with some RNA-seq data, and found the detected adapter sequences are identical to some rRNA or mtRNA sequences.
For example:
Detecting adapter sequence for read1...
…
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Hello Torsten,
Firstly, thank you for a great program! I have a question about the --rfam option. This picks up ncRNAs with Infernal - this invocation of cmscan is not used to look for 16S rRNAs or…