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Primer erosion with omicron is showing itself to be fairly important. In some cases, masking problematic amplicons may help improve the interpretability of the data. Is it worth bringing that out here…
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The *amplicon* prep sheet includes info on the robots used for various steps of the sample handling. The shotgun process includes a compression step (which the amplicon process does not) for which La…
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Separate from Primer plate selection, for each plate
Raised in amplicon walkthrough 07/30/2018
(Note that currently date of working primer plate is stored as part of working primer plate name)
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accumulate papers describing oligotypes and amplicon sequences and exact sequence variants
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Dear Maintainers,
I got this error when trying to run ampliconsuite inside nextflow, so I am unsure if this occurs because of nextflow or maybe the issue arises on your side.
I also tested the con…
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**Improvement Description**
Typically we use hidden actions for the inner part of a parallel pipeline loop, e.g.
```python
def some_parallel_pipeline():
...
collection = partition_data()
f…
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Hi @jluebeck ,
I have three questions regarding the classification of the amplicon.
1. There are some differences (see image below) between the current version and the legacy_natgen_2020 (from t…
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HI:
I use the jacusa2 to run my amplicon data, but the result lost the last position of the ref, that is, if my ref is 182bp long, the jacusa2 result only print the 1-181bp. The parameters I used…
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Dear Mr/Mrs,
I am trying to combine data from two PacBio runs: DNA (10kb) + cDNA (10kb), these regions overlap each other partially and I would like to phase them based on short-tandem repeats and …
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I am new to metcat and attempting to assemble amplicon based sequence reads for a virus genome. I am wondering on the set of optimal parameters for a 16000 long genome with 1500-2500 long amplicon seq…