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Hi,
I am checking MHL values of my BAM files and see something unexpected. The sample BAM I can share holds alignments of amplicon bisulfite sequencing of five regions to a very high depth (5000-5000…
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Hi,
I am just testing the tool on my FASTQ files and always getting an error "buffer overflow detected". What does it mean?
Do FASTQ files need any preprocessing (e.g. a header change)?
Sho…
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**Describe the parameter code you need**
Metabarcoding datasets usually come with relative abundances in terms of sequence reads per ASV or OTU. This is a request for terms for:
- Total abundanc…
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I am trying to run the pipeline on some targeted amplicon sequencing by using the recommended settings (by using eval as target eval and providing a .bed file).
The pipeline runs fine if I use
sn…
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Hi,
I am trying to run strelka2 in somatic mode on high depth data (~3570.95X) on a small gene panel (trusight) but the job never ends. I killed the job after 15h.
This is the command line that …
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Hello,
I hope that you can help me with this :
Can I use nanocaller on my fastq.gz files generated using ONT minion technology ?
Can I use it to detect variants in fungus ?
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Dear authors!
Good day to you.
I was wondering how I can use SWAMPy for other pathogens? Which parts of the code should I modify?
I have different reference genomes and primer sets. Is there any …
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i am new in this field , i am not able to install and run ARGsAP PIPELINE 1st stage.I have Ublastx_stageone2.3 zipped file and extracted it too. which command to use for further proceedings. please h…
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Reply with learning objectives (and challenges to test them, if possible).
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Can I request 5' adapter/primer trimming (forgive me if I missed it in the docs):
e.g. If a primer sequence is AACTGCC:
```
Before: 5'-aatgaAACTGCCactgggagagagccatcattgatcgtagctg-3'
After: …