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(a.k.a custom hints or custom requirements)
- [ ] Seven Bridge
- [ ] main: https://github.com/rabix/bunny/wiki/CWL-draft-2-extensions
- [ ] https://docs.sevenbridges.com/docs/list-of-executio…
mr-c updated
7 months ago
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Hi,
I'm trying to reuse some relatively "old" illumina RNA-seq data not strand-specific. What would be the best way to process them?
Thanks a lot
Francesco
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Dear developers,
I am a bioinformatics student, and I plan to perform QC for many NGS reads data from several different sequencing platforms such as ILLUMINA, LS454, ION_TORRENT, and ABI_SOLID. You…
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I have Illumina novaseq, pair ended reads and I want to trim the non-internal adaptors from both ends. The adaptors I used for library praparation are NEB next adaptors for illumina. So I executed the…
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Hi all,
I usually run `unicycler` on minion + illumina reads but got some raw reads from pacbio the other day and wanted to try it. I used `bam2fastq` to get fastq files from the pacbio bam files, …
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We should dig into this a little more and how this matching occurred. I feel like something went wrong here but I need more time to determine whether this was due to our coordinates or due to somethin…
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The read structure `75T8B75T` works but `8M67B75T` does not. The error I get is:
```
Exception in thread "main" picard.PicardException: Don't have both phasing and prephasing values for tile
at p…
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relates to https://github.com/microbiomedata/nmdc-runtime/issues/249
For some of the records the ingest code populated `instrument_name` with `Illumina Illumina HiSeq`. This is because some of the NE…
aclum updated
11 months ago
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Dear author,
There are very high ratio unmapped reads for **'too short'** and **'other'** while mapping to `T2T chrm13` genome, but **it worked for hg19 genome**. BWT, there is a **83% reads mappi…
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Hi,
First, I'm aware that h5 is deprecated, but I'm trying to install a package (velocyto.R https://github.com/velocyto-team/velocyto.R ) which has h5 as dependency...
I have this problem whil…