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We are using the scripts which you published on this paper and repeat the m6A calling using EpiNano, with the datasets published on Nature Communication (2019) including Curlcakes and yeast datasets.
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TTT16 updated
2 months ago
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Thank you for this amazing resource. On the AWS landing page it sounds like there are tissue samples processed but I only seem able to find cell lines sequenced?
Apologies if this is just me missun…
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Hi nanom6a team! I noticed that you used m6A sites identified by miCLIP from public data (Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification) in Fig…
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I tried to use this tool for Nanopore direct RNA sequencing. However, library type was not recognized.
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Hi!
I try to run RATTLE with my fastq reads (direct RNA sequencing):
`./rattle cluster -i my.fastq -t 24 --fastq --iso --rna -r 5
`
I got an error:
what(): cannot create std::vector larger…
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while processing the data with Tombo before using MINES, which reference fasta should be used for ONT direct RNA sequencing data - genome or cDNA fasta.
akk01 updated
3 years ago
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Hi,
I am interested in using long and short reads to assemble a transcriptome with RNA-Bloom2.
I had a few questions regarding pre-processing of reads before using RNA-Bloom2.
I am loosely fo…
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Dear Peng,
I wonder if it is possible to use Deepsignal to detect methylations in RNA from samples sequenced by the direct RNA sequencing kit from ONT.
I think about training a model by using comp…
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Dear Greg - thanks for your continued work on this and for this follow up paper with updated pipeline. I'm the lead author of [Hermida et al. Nat Commun 2022](https://www.nature.com/articles/s41467-02…
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Hello Team,
I have a basic query regarding the fastq reads , i don't have fastq reads in pairs, i have single fastq reads generated from direct rna sequencing(SQK-RNA002), is it possible to run DIC…