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**Format name:** < FAST5 >
**Short description:** < The FAST5 file format stores the raw electrical signal levels measured by the nanopores in flow cells for Oxford Nanopore Technologies sequencer…
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Hi,
I am interested in using long and short reads to assemble a transcriptome with RNA-Bloom2.
I had a few questions regarding pre-processing of reads before using RNA-Bloom2.
I am loosely fo…
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When basecalling RNA2 data with dorado 0.5.3 it performs automatic read splitting via an open pore detection algorithm.
This algorithm is broken, as it not only detects actual open pores, but also…
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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?
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Hi,
Thanks for the useful variant calling tool for long-read RNA sequencing data. Since the entire tool and the evaluation process are developed in R language, it may be difficult for users who a…
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When calculating depth by GC content, samtools stats command ignores reads overlapping boundary between two GC bins. For short read data, it doesn't matter since, as explained in [stats.c](https://git…
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Dear bcbio developers,
For some of the species that we work on we are now starting to receive significant numbers of long read sequenced samples.
The samples are sequenced using nanopore, PacBio …
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Can DIPAN be used in long-read transcriptomics such as nanopore reads?
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Dear Sirs, I have some problem about tmp file which was not created. I ran the job in linux server. There is some error in below:
passedQC_iF02_iR09.fasta contains 17 reads.
--> Low number of read…
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Hi
Thanks for developing this tool.
I now have resequencing data for two parents: **P19_clean_1.fq.gz,P19_clean_2.fq.gz,P48_clean_1.fq.gz,P48_clean_2.fq.gz**, and the offspring ONT (R10.4.1) data: *…