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Hello community,
I recently performed a metagenomic analysis on sequencing data generated from a zymomock community by Nanopore Sequencing (MinION) and wanted to assess the sequencing bias by using…
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I'm processing our first Element AVITI 16S amplicon sequencing data, using dada2 in QIIME2. I'm wondering how to do this optimally, as it appears that it behaves a bit differently from MiSeq data in d…
mniku updated
1 month ago
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Hi @bluenote-1577,
I have downloaded Illumina sequencing data of the ZymoBIOMICS Fecal Reference.
Then, I have performed taxonomic profiling with sylph (database: gtdb_r207, parameters: --estimat…
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Hi,
We are trying to detect chimeras in some nanopore 16s reads. Although we already know that there are some chimeras in our reads, we are not able to detect them using the parameters from the yac…
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This is my first time using DADA2. I followed the DADA2 tutorials as well as the inputs from https://github.com/ErnakovichLab/dada2_ernakovichlab to process a mock community sample sequenced for 16S V…
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Hey Simone,
I was wondering if you're planning to update your wonderful pipeline to the new kit12/14 chemistry, flowcells and super accuracy basecalling? :-)
With best regards,
Tamara
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HI again,
I ran my first 5 real samples using your nice tool in docker mode (the example run succeeded before this one)
The samples are 5 identical Zymo DNA community standard D6305 run with diffe…
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Dear Developers,
Greetings from India,
Thank you for providing such detailed documentation for the users. Being a novice to the Bioinformatics world, even I was able to install the S/w and execute…
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## Bug Report
Hi GATK team,
I've just been running some variant calling benchmarks on a set of bacterial isolates and I was trying to understand how GATK HaplotypeCaller chooses which re…
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Hi readfish developers,
Can you tell me which sequencing experiment was performed to generate the fast5 file - [PLSP57501_20170308_FNFAF14035_MN16458_sequencing_run_NOTT_Hum_wh1rs2_60428.fast5](htt…