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# Possible reasons for duplex reads' quality are low
Hello, thank you for increasing the read quality! We always observe higher read mean quality on duplex than simplex reads, especially simplex pa…
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hello,
I am starting with PacBio amplicon libraries generated by a colleague which were demultiplexed using lima. Could you describe the best workflow for this starting point? Thank you.
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```
$ abricate
SYNOPSIS
Find and collate amplicons in assembled contigs
AUTHOR
Torsten Seemann (@torstenseemann)
```
Crap, did I download the wrong code? I was looking for the antibiotic …
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One issue we should check is whether the primers used in the amplification match the 5' ends of the sequence. PI mentioned that some custom amplicons may not match up
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Dear Developers,
I'm trying this pipeline. However, when I typed
python ./scripts/Master.py -h
, it turned out:
Traceback (most recent call last):
File "./scripts/Master.p…
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Hello Kristoffer,
I ran `NGSpeciesID` with the following command:
```
for file in *.fastq; do
NGSpeciesID --t 4 --ont --fastq $file \
--consensus --medaka \
--abundance_ratio 0.005 \
--rc…
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hello, i want to install this software,but i meet this question as follows,please help me to solved its.
bam_ampliconclip.c: In function ‘amplicon_clip_main’:
bam_ampliconclip.c:639:23: warning:…
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Strangely affects CMV amplicon of gene UL97.
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Problem with fungal amplicons. Read quality is generally lower for longer amplicons and split_libraries_fastq.py in QIIME doesn't appropriately handle trimming during quality filtering of length poly…
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When running "hitman_deploy," I am getting the error below. Any suggestions?
Ken
```
[hpc:service0@/rsgrps/bhurwitz/hurwitzlab/tools/hitman/scripts]$ ./hitman_deploy --dir /rsgrps/bhurwitz/hurwitzl…