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Hi,
Not sure if this is an issue, or the problem is sitting in front of the computer.
We finished sequencing our first genome using the nanopore MinION and I am currently trying to assemble it.…
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Hello Simone,
I installed the program on Ubuntu 20.04.3 LTS following the instructions and I tried initially to create the database with import_database.sh but using ncbi the process is killed, perh…
asogg updated
2 years ago
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Hi,
I'm trying to use your DNAscent software to call BrdU in Nanopore data we have being producing lately. I have followed you manuals to install and your workflow to map the data and produce the D…
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Starting script: perl /home/aalafiatayo/miniconda3/envs/SqueezeMeta/SqueezeMeta/scripts/SqueezeMeta.pl -m coassembly -p analysis_results -s nanopore_data.txt -f nanopore --miniden 60--minion -t 12
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I have nanopore reads from minION and promethION flowcells (both R9) from the same individual. All reads were basecalled with the same guppy version (but different models for minION and promethION rea…
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This is just an issue to track progress on the idea.
The basic proposal so far is some form of side-channel in a SAM/BAM/CRAM tag which is used in conjunction with the sequence field to characteris…
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Hi,
I'm having problems with canu not finding any overlaps during the mhap step. The dataset is nanopore reads basecalled using Guppy and the SUP model, adaptor-trimmed using porechop and filtered …
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The current config seems to be set for the ARTIC V1-4 primer schemes:
https://github.com/nf-core/viralrecon/blob/f0171324a60d1759ca7f5d02351372d97d22cc12/conf/modules.config#L54-L55
This results i…
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Hi,
Thank you for providing the Sicelore software. I have been using it to analyze some of my data.
I realized I was losing a lot of reads (60.8%) at the polyA scanning step. Starting with 19,83…
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Hi,
I am trying to run the following command with the default python being set to 3.8 instead of 3.9 (since the Guppy backend needs python < 3.9):
megalodon /Users/rajats/Documents/Projects/MinI…