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https://mp.weixin.qq.com/s/TXO7Z9bxxhUhhox1VwA_LA
ixxmu updated
2 years ago
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Hi everyone,
If you have any issues with the code, please post them here. We will get back to you as soon as we can. Thanks!
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Hello,
I am running SqueezeMeta on Nanopore reads in the sequential mode. The commands are:
SqueezeMeta.pl -m sequential -s sample_file_EXX001_.txt -f EXX_fastq --minion
For the canu step, …
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Dear @adnaniazi,
What do **NA**s most likely represent in the polyA tail estimation? Can we speculate that they are typically longer or sorter than the population?
Thanks for developing this gr…
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Hello,
I am trying to use of the `--mod-binary-threshold` option in Megalodon's mod base aggregation.
It is described as (probability of modified/canonical base) and has a default value of 0.75. M…
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I ran NanoCaller on ONT release 6 NA12878 data with the default parameters (NanoCaller1). Reads were aligned with minimap2 and all alignments were kept. Reads were filtered for the quality score 7, fi…
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I am trying to get canu-correct to run as quickly as possible on ONT MinION data. So far I am downsampling the input file so that it has less spikey coverage, and also tweaked canu's parameters to the…
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Hi,
I'm attempting to correct very high coverage amplicon reads generated by minION and can't figure out the specific reason it keeps failing even after scouring the logs. I need to make sure all rea…
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Project: ENCODE
Oxford Nanopore has developed a number of small affordable sequencing instruments that allow labs to sequence their own samples without sending them to sequencing centers that run m…
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Hi,
I'm trying to dereplicate minion sequences that have already been processed for quality, obtaining a fastq.gz file.
I would like to dereplicate those sequences mimicking the ASV approach. I trie…