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Hello, thank you for your advice in #238 .
In my case, the phenotype was population defined by population structure analysis(like lineageA vs lineageB).
In phenotype file, binary (0,1) values wer…
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Hello, I tried to performe unitig annotation using annotate_hits_pyseer.
In the pyseer tutorial, it was written that annotations marked with ref can partially match between k-mer and reference sequ…
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Hello, I am having an ONT problem--this might be related to Issue #474 . The program runs fine with only using the HiFi reads. My script is basically identical to #474 :
`hifiasm \
-o tx3362.asm \…
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Hi,
Considering the fast assembly of this software, when I provide the mitochondrial genome as "-s", the mitochondrial contigs can be quickly assembled?
Deyuan
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Hi!
Sorry for the inconvenience, I think I found a bug.
I was computing matchtigs for the sequence SRR332538_1, k=21.
The program failed to check the assertion `has_breaking_edge`.
I got the warn…
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Dear all,
Thanks for creating metaMDBG :). I am trying to generate an assembly graph (.gfa) after running metaMDBG. Unfortunately, a
`segmentation fault (core dumped)` is raised. Here is the com…
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Hello,
I am assembling ONT R10 LSK114 Reads of cultivated bacteria with flye (2.9-b1768). When I'm inspecting the assembly.gfa in Bandage, there is one more (short) contig than in the assembly.fasta.…
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Hey,
I have been using your great software for almost a year now. I used it to index kmers and their count of 1000 human WGS samples. I am also going to use it to index cattle WGS samples.
I am foll…
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Hi!
I'm having trouble processing a bcalm file with 12,585,517 nodes and 14,600,062 arcs (sequence SRR061958_1, 21-mer minimum count 1). I don't have enough RAM even if I've allocated 330GB for this …
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Hi @jnalanko , I tried to test themisto with [101 microbial genomes](https://1drv.ms/f/s!Ag89cZ8NYcqtlAgLM6h1X7JtYB_L?e=nix6e5), but it crashed in the step of Sorting KMC database. The disk has enough…