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There is no hurry for this as Andrew is on his placement at SciBite , but during the RNA Central meeting I said we would export the publications to see if they could be used to improve the gene summ…
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Thank you for this amazing resource. On the AWS landing page it sounds like there are tissue samples processed but I only seem able to find cell lines sequenced?
Apologies if this is just me missun…
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Hello,
Can the same pipeline be used for cDNA sequencing as well? I have the fastq reads and the reference genome and transcriptome fasta sequences.
Thank you.
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I am trying to obtain m6a modifications from direct RNA sequencing of the avian influenza virus. It seems I have been able to obtain the eventalign.txt file... but after running m6anet dataprep the o…
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Hi Jared,
I am using eventalign to align signals from direct RNA sequencing.
However, as the RNA that I am sequencing is expected to have a signal that is different from the current in the kmer mo…
awjga updated
5 years ago
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Hello! I'm trying to run `eventalign` for a dataset of small oligonucleotides of size 100nt, but most of the reads fail with either "no alignment" or "not calibrated".
This is the command that I ex…
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Hi @jts,
I am currently running `nanopolish index` on two direct RNA libraries, one obtained from a MinION and the other from a PromethION. The former seems to have worked perfectly fine when inclu…
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Dear Greg - thanks for your continued work on this and for this follow up paper with updated pipeline. I'm the lead author of [Hermida et al. Nat Commun 2022](https://www.nature.com/articles/s41467-02…
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Hi,
FASTdRNA is an easy-to-use workflow for processing direct RNA sequencing data. It makes the processing flow simple and lets the analyzing steps be done consistently, saving users a huge amount…
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Hi,
I am interested in using long and short reads to assemble a transcriptome with RNA-Bloom2.
I had a few questions regarding pre-processing of reads before using RNA-Bloom2.
I am loosely fo…