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Hi,
In version 0.15.0 seqkit amplicon only keeps one amplicon (the largest) per primer pair. I don't always care about the largest amplicon.
Would it be possible to implement the feature of kee…
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editor preferred term: Roche 454 GS FLX+
alternative terms: 454 GS FLX+
textual definition: is a DNA sequencer which is manufactured by 454 Life Science Corporation and enables pyrosequencing to be …
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Dear Mr/Mrs,
I am trying to combine data from two PacBio runs: DNA (10kb) + cDNA (10kb), these regions overlap each other partially and I would like to phase them based on short-tandem repeats and …
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**hi,thanks for your great software!
When I plan to use full mode to design primers and check primer specificity, or check primer specificity, the progress bar of the task will run all the time. I …
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The workflow could be run on all amplicons only (and not specific target SNPs aswell). If we can reduce the number of inputs for some users that's a bonus.
Things like allele frequencies and cover…
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I amplified SARS-CoV-2 positive samples with the ARTIC scheme V3.
Additionally I amplified three longer amplicons (2.5 kb) to cover regions of low coverage with the ARTIC scheme.
I tried to create …
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Hi there,
I have two questions:
1. You have mentioned that "ONTbarcoder has been optimized for protein coding gene like COI. While **there are ways** to obtain consensus for length variable non …
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### Feature Request
This issue is a solicitation for feedback on an idea.
I've been working on the [RSV pipeline](https://github.com/CDPHE-bioinformatics/CDPHE-RSV) and reusing tasks from this r…
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Hi,
I am really struggling with convector. I try to launch pipeline.py but results me in some errors so I tried a step by step procedure. I have a folder of BAM files and a .bed file correctly format…
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In the AmpliconClassifier documentation is detailed, that only cyclic amplicons can be classified into BFB or ecDNAs.
However, in my analysis, I noticed that some were classified as Complex, but the…