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I observed that in the current version, the mean of the quality values at each read position is identical between first and second read. This is not the case for the reads I used to train the quality …
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# Issue Report
## Please describe the issue:
I am aware that there is no plan to support dorado on AMD GPUs, e.g. [I](https://github.com/nanoporetech/dorado/issues/952), so I am sorry to raise t…
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I am trying to create a custom PoN file by using a following command, however it outputs an error message about 'sort' without generating a PoN file. Could you please advise on this issue.
Command …
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### Operating System
Other Linux (please specify below)
### Other Linux
RedHat 7.9 (Maipo)
### Workflow Version
v2.9.0-gd9e8593
### Workflow Execution
Command line
### EPI2ME Version
_No resp…
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to facilitate custom basecalling
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Dear m6anet-team,
I have a question regarding m6anet, which I try to use in Galaxy to reproduce some RNA-sequencing study in order to get familiar with this topic.
In your manual you write for dat…
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Can I use this version of Guppy to base call reads that have r10.4 flow cells and e8.1 enzyme? I believe the guppy base caller version was v5.0.15
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Hi~ I am trying to train a bonito model from scratch.
To obtain my training data, i basecalled the reads with the command as below:
```bash
data_path="~/codes/bonito/data"
key="Acinetobacter_bau…
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Hello,
Love using deepbinner for basecalling and demultiplexing, but we're starting to use the second set of barcodes that ONT has started selling. Any chance of support for NBD-114?
Thanks for the …
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It runs well in the non-basecalling, non-demultiplexing, non-alignment pipeline. However, it not runs well in the non-basecalling, non-demultiplexing pipeline. The test dataset is from nf-core/t…