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Hi Nanopore team,
I noticed a significant (~10x) slow down of the Dorado basecaller when adding the 6mA modification model. This is notable since adding the 5mCG_5hmCG modification model have almos…
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Hi
With a recent update nanopore is now producing a new format. Is this also accepted at the ENA? (https://ena-docs.readthedocs.io/en/latest/submit/fileprep/reads.html#oxford-nanopore)
Best reg…
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Hello,
Our lab has been using hifiasm for a number of genome assembly projects. We are using Nanopore reads (kit 14 chemistry, R10.4.1 flow cells) that have been error-corrected with PECAT or HERRO…
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Thanks for providing and maintaining HASLR. Is it possible to use HASLR to assembly sequences from pacbio, nanopore, and Illumina all together at the same time? I have used HASLR to assembly pacbio …
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Hello,
I only have one fastq file for the sample. When running the amplicanPipeline function I get an error that my forward file is blank, but my forward file column contains the name of my fastq f…
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Hello,
I have sequences of RNAseq generated using both nanopore and illumina, I want to know if it is possible to give in input to RATTLE both sets?
Thanks
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Hi,
I wanted to quantify reads sequenced with a Oxford Nanopore MinION using salmon 0.12.0. With activated --validateMappings option mapping rates were below 1% (mapping them to the human genome us…
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Dear community,
According to the manual of MITObim, this useful software can use PacBio long reads for mitochondria assembly. Can I use Nanopore long reads, too? Thank you.
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Hello,
I am trying to use FLAIR to look at transcript isoforms of FCGR2C with Nanopore cDNA sequencing. FLAIR appears to be failing to pick up isoforms showing retention of the final intron (which…
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[concatenate_read_example.pdf](https://github.com/willros/nanomux/files/15457608/concatenate_read_example.pdf)
Hi William,
How do you define where do the barcode starts and ends in the read?
I fo…