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Hi, Jamshed @jamshed
Thanks for cuttlefish giving me a new way to build cDBG in a very fast speed. I have one question after reading your paper. I noticed that you find the maximal unitigs by trav…
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`MBG bioconda 1.0.16`
Hi,
- I am testing MBG on an organellar genome and noticed the results can change if you run the program multiple times. I couldn't find a seed parameter in the options to …
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Hello!
I see, that two nodes in unitigs graph (gfa) are not linked by an edge. At the same time this nodes are merged togehter in the primary assembly...Why there is such inconsistency between unit…
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I am very interested in using cuttlefish from within C++ code and was wondering if it would be possible for you to provide some kind of C++ API / header library? I'd be particularly interested in func…
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Dear John,
I’m using unitig-counter/1.1.0 and then feeding the output into pyseer for LMM-based association analyses. However,
I’ve noticed I get different number of significant unitigs when usin…
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The below is an example: the two chromosome-level haplotypes are continuous on the unitig graph, but in the final `hic.p_ctg`, one unitig (charactered as “A”) is breaked.
![hifiasm](https://user-imag…
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I'm aware questions are preferably answered at Biostars, however, I saw older questions not being addressed there while being here. If this is not convenient just notify me and I will immediately tran…
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Hi,
I have executed pyseer and I would like to map my unitigs not to the list of my input files but back to just one fasta/gff file that represents my pangenome.
This file contains representat…
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Hi Developer,
Thank you for developing this good tool.
Is it possible to use purge_dups to get the best set of contigs from two different assembler?
For example, I got draft genomes of the same org…
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Hello,
We are making a hybrid assembly with MaSuRCa, we have launched it twice changing some parameters of the configuration file, both times it has stopped because of the same failure in the creat…