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貼吧活動：(請查閱 [SARS-CoV-2 Timeline by 2020.02.21](https://github.com/agorahub/_meta/blob/agoran/theagora/sari/Memorandum_2020-02-21_SARS-CoV-2-Timeline_Nathan.pdf?raw=true), by Nathan :cloud: )
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Hi, I am using primer3 to design primers of nested PCR for the next generation sequencing.
For example, I would like to sequence a gene which is about 10000 bp long. Assume that the first pair of pri…
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Hi Matt,
This is only thematically-related to inStrain. If you don't have the time to answer this, no worries; I would simply close out the issue. If you do:
Colleagues will be performing field …
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Hello! [This is the paper I am using](https://onlinelibrary.wiley.com/doi/10.1002/edn3.374) to mimic the Nanopore process - this is the most clear of the 3 I was cobbling together. It looks like I sho…
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Hi Ben,
I've recently been working through the big data PE workflow. This afternoon I updated dada2 to version 1.8.0. Since the update the script cannot complete the mergePairs(..) function.
I …
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Hi,
I'm running shapemapper2, and it's taking very long although I'm using 45 threads. Below is some context on my run.
I have my gzipped files with the following sizes:
Modified: R1 ~1.8 GB, R…
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I am testing lotus2 on my amplicon sequencing data. I used lima for that but I don't like its demultiplexing report. I read your tutorial but didn't find anywhere stating if mismatches in barcodes can…
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Hi all,
I am running an analysis on Naopore amplicon data (without primer processing, since it is a multiplex study, which I want to trim and treat further downstream) but get the following error …
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Hello,
I am trying to decide on whether the new version of MIXCR is compatible for our TCR seq pipeline.
Our TCR Seq pipeline is based on the paper "RNase H-dependent PCR-enabled T Cell Receptor…