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editor preferred term: Illumina NextSeq 500
alternative terms: NextSeq 500
textual definition: Desktop sequencing system to analyze whole genomes, exomes, and transcriptome.
definition source for t…
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Hi,
We have some tools that expect to see allele depths in FORMAT column (ie A,C,G,T or AU,CU,GU,TU) and TIR/TAR for indels. Would be great if there was a way to get these in the vcf output.
Tha…
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Dear Dengfeng,
Thanks for your tool.
I tried to use purge_dups to clean our assembly. I have some questions:
1. we have Pacbio sequencing data and Illumina sequencing data, I want to use all of …
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Download reads from [Sequence Read Archive (SRA)](https://www.ncbi.nlm.nih.gov/sra) for an organism with a good reference. If that has a high SNP count then it is a pipeline issue.
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We have a few paired samples that for some reason have different platforms in each bam of a given pair. As a result we got the following error:
```
BAMs have different sequencing platform: ILLUMINA,…
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formatting problems with externally supplied ("walkin" ) keyfiles, such as ragged ends and non-ascii characters, was previously handled by the script sanitiseKeyFile.py in this repo, which was called …
afmcc updated
10 months ago
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Dear developers,
I am a bioinformatics student, and I plan to perform QC for many NGS reads data from several different sequencing platforms such as ILLUMINA, LS454, ION_TORRENT, and ABI_SOLID. You…
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I have assembled few bacterial complete genomes using nanopore long reads via Unicycler bold module followed by multiple rounds of polishing by pilon using Illumina Miseq reads.
While submitting the…
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Hi,
we're planing to set up an instance of MISO in our own sequencing department soon, but amongst others we also have an Illumina sequencer type which uses a different style of [sample sheets](htt…
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Trimmomatic fails for sequencing data generated by the Element Biosciences AVITI instrument if Phred33/64 encoding is not provided (quality score is autodetected for Illumina data).
"Error: Unable…