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I want to ask if there is any possibility of using WHATSHAP for phasing my dataset which consist of Illumina whole genome sequencing at high coverage using also long reads from PacBio and Nanopore.
I…
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It would be very useful to report the total number of reads alinged to viral genomes and the fraction of reads aligned to each viral genome with respect to the total number of reads in a sample.
The…
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I have assembled few bacterial complete genomes using nanopore long reads via Unicycler bold module followed by multiple rounds of polishing by pilon using Illumina Miseq reads.
While submitting the…
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Hello community,
I recently performed a metagenomic analysis on sequencing data generated from a zymomock community by Nanopore Sequencing (MinION) and wanted to assess the sequencing bias by using…
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Hi,
I just, want to know that it only works for genome sequencing, not for RNA sequencing. So are you planning to do an RNA sequencing simulator also included in this tool?
Thanks in advance
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Hello @ibn-salem @priesgo @ozlemmuslu @martinloewer @mostafiz2010
My data is 260 million distinct sequencing reads(2×150 nt).When I predicted GFs with the Easyfuse 1.3.6 from my data, the software ra…
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Hi Yan,
I ran the test scenario and I ran your test script and I ran the tool on a fasta that contains only one fasta-record with an assembled chr22.
Both lead to outputs. (is am interested in res…
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Hi, I have a question:
my config.txt is like:
********************************************************************************
Project:
-----------------------------
Project name = Te…
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**Develop an interactive application to facilitate informed sequencing quality control decisions for downstream analysis on many samples**
There's the saying of "garbage in, garbage out" in compute…
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I tried the program on PacBio hifi reads, which were for amplicon sequencing of some plant DNA samples, barcoded on both ends. The reason is that LIMA generated very simple report missing a lot of inf…