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Support the first step of demultiplexing without appealing to CASAVA
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Dear Qiimer, I know it is common that illumina 16S sequencing does not include primer sequence but it will be nice to have this option to enable it? It is not convenient to using the parameters in spl…
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Hi, I have HiFi data from the low input protocol sequencing.
Ran genomescope v2 online after:
```
KMC/bin/kmc -k21 -t5 -m64 -ci1 -cs10000 @cat.files cat.kmcdb tmp
KMC/bin/kmc_tools transfor…
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Marc,
I'm trying in vain to import my own manifest (version b5) with methylprep. Can you give me more explanation about this.
BR
Stef L
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1. Include our published MDS/AML .meta files in the example data set
2. Run the 1000G Illumina Omni 2.5 data set through Genvisis to create imputation ready VCFs
3. Impute to the TOPMed reference pa…
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Good day,
I am very new to the bioinformatics field so my apologies if this question/issue is not posed optimally. I am trying to run the pipeline (MacOS) on illumina fastq files and Jobs 1 & 2 ar…
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Samclip calculates alignment end position as alignment start position + length of read.
`my $end = $start + length($sam[SAM_SEQ]) - 1;`
This works fine for Illumina data, but often falls short …
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I had asked a similar query in pipeline slack channel 6 months ago as I don't have the requisite data. Now Pubudu has same query - time to address this once and for all:
Ref: https://docs.google.c…
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Dear @alekseyzimin,
I am running Masurca and having as an input: Illumina PE reads + Nanopore long reads.
I ran the masurca with the same configuration file twice (in two seperate folders) and …
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I use alfred to extract reads aligned to a particular region from a bam file. My bam file is derived from Illumina paired-end read data. Currently I can extract all reads mapping to a particular locat…