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Hi,
I am having trouble with running Sequence quality control using DADA2 in `DADA2: 1.26.0 / Rcpp: 1.0.12 / RcppParallel: 5.1.6`
My script is as shown below:
`run_dada.R --input_directory /tmp…
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Somehow I'm ending up with a very low number of duplex reads after using Dorado duplex basecalling with the following command (used the ligation sequencing kit V14 SQK-LSK114):
`dorado duplex dna_r…
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Hi,
What parameters do you suggest changing when working on Illumina short-amplicon reads of COI genes?
For example, would you:
- `SelfConsist = TRUE` instead of proving 16 × 41 Transition prob…
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Hi there! I executed the steps listed in the readme to create a conda environment and install RibDif2 and its dependencies, then tried to run:
ribdif -g Ruegeria
But I get the following output,…
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We have examples where there is an amplicon with two right-hand primers. Neither primer is excluded at the primer/amplicon identification stage, and both are provided to cylon.
Observed behaviour: …
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hi，how to resolve this problem ？
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Writing to output...
Primers combination number 1
Number of written amplicons: 272
Sorting amplicons to multiplexes 1_2 (…
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Hello!
I'm experiencing some issues with RAMPART and I'm wondering if there is a solution available. I would like to know if anyone has been able to resolve similar problems.
In our laboratory, …
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Options:
- [ ] Only Amplicons vs. semi-Amplicons: with only one primer hybridize due to partial sequences
- [ ] Min and max length of the amplicon
- [ ] Ta and max delta-G
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In the fct find_pcr_products, the products that lies outside the minimum and maximum product size is filtered out.
This could potentially remove relevant PCR products that lies just outside the boun…