-
In my recent usage of HIBAG, I have hla typed genetic data called from the hg38 reference genome. Though I was able to lifotver the hapmap training data positions, I believe it will be much more accur…
-
Dear SqueezeMeta developers,
Recently I requested the SqueezeMeta installation to the Admins of the Supercomputing center we use, but when I got ready to use the SM tool two errors appeared after…
-
Hi,
I'm having an issue running igblastn. Every time I do, I get an error saying an annotation database in internal_data could not be found.
I did follow the installation instructions.
igblast is…
-
Not sure if already known... I did not see a similar issue so I just write it up for the next release ;)
- Affymetrix - log in required - is that intended, shall we keep that?
- Agilent, Illumina …
-
A pileup of uncorrected Guppy v3.6 reads produces a better guess at the true sequence compared to corrected reads. The errors are in deletion variants and only in specific locations; for the most part…
-
Hi,
I incidentally poked over your project and I wonder why you keep track of `Average read depth` and of `Observed insert size`. The former would be better replaced with `Median read depth` and th…
-
Base calling, is the systematic assignment of nucleobases to chromatogram peaks in case of electrophoresis, current changes resulting from nucleotides passing through a nanopore in case of Nanopore se…
-
Hello
Using graphmap 0.5.1 I want to overlap illumina MiSeq reads derived from enrichment sequencing. The reads are enriched for a large gene family. I would hope for large numbers of relatively shor…
-
Hello Developer,
Thank you developing this tools.
I have a question regarding insert size plot generated from QC.makemultiplot.pdf
Why does it have two peaks?
below is my command and p…
-
I think this would be a useful feature as `--usearch_global` only aligns a small percent of my reads because I haven't truncated the database to just the region that we sequenced.
We sequence using n…
audy updated
10 years ago