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Hi,
I'm working on a plant species that does not have sex chromosomes and should be highly inbred (homozygous). I'm curious about why the haplotype assemblies are smaller than the primary assembly…
pjm43 updated
2 years ago
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Quast report of merged_canu_hifiasm_hifi.fasta
N50 28033498
command:
nucmer -l 100 -prefix out merged_canu_hifiasm_hifi.fasta merged_canu_nd_racon_ont_hi…
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Hi Yoshi,
I modified the run_hifiasm.sh to add the "--hom-cov 148" argument for the sample where homozygous coverage was inferred incorrectly by hifiasm. I also changed the $OUT_PREFIX variable so th…
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Hi @arangrhie,
I got `Merqury` to work and obtained fairly good results for my human individuals. However, other than the standard plots how can I get the blob plots showing the "correctness" for t…
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Hello,Professor
I plan to use minigraph to construct a pan-genome graph for two genome assembly generated by hifiasm. My command is : `minigraph -xggs -t16 B.all.fasta B.all.fasta > out.gfa`. I am…
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After using hifiasm to generate partially-phased assemblies (the `bp.hap?.p_ctg.gfa` files), I am confused about regions where the assembly is apparently missing for one of the two assemblies.
For …
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Hello,
First of all, this is really useful thanks for this tool.
I'm trying to use it on Hifiasm graphs. I saw issue#35 and while waiting for correct cigar as output of Hifiasm I implemented a s…
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In a recent study [https://doi.org/10.1093/bib/bbac146](url) , the authors found that the post-assembly correction process (pilon) improved hifiasm assembly quality. I wonder whether it is neccessary…
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Hi,
We are struggling to perform de novo assembly of meta bacterial samples selectively cultured with antimicrobials from wasterwater using hifiasm-meta with the default parameters. The sequencing de…
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Hi xfengenfx
recently，i use the hifiam-meta to assemble my metagenomic HIFI data，i encountered same error in two times at two compute
cluster，which shows stop at the Writing GFA step suddenly. h…