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Hi,
I am trying to use the STARsolo function to process full-length 10X cDNA reads. I parsed the data into read pairs (One containing the actual (~1200nt) read, the other the 10X cell barcode and …
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Hello Alex,
So this is supposed to be V2 5' assay. So I am wondering if the parameters are correct. Is the whitelist different for 5' assay? I am wondering if that is causing an issue.
Here i…
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Hi!
I am using the Han et all preset (han-et-al-2014) , with mixcr-4.3. I am facing several issues:
1. Low coverage of the plates (I have 20 plates in total, 5 sorted cells in each well, librar…
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I am new to demuxlet and running demuxlet as part of demuxafy within a container:
singularity exec --bind /project/rrg-gturecki/Sarah/multiplex_test_demuxify_run2_cecum_poolC/outs/raw_feature_bc_ma…
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# Issue Report
I have previously basecalled data with the Rapid Sequencing kit (SQK-RAD114), and I sequenced now some samples with the Rapid Barcoding kit (SQK-RBK114.24)
I ran
## Run environme…
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Have you used bcr_dist with non 10X data? I see you can load a "BD" file, I am not familiar with this format...
Hoping to be able to map the emerging AIRR Clone format (https://github.com/airr-comm…
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Hi,
I am using live stream for detection of barcodes via camera on the phone.
High end mobile devices does not have a big problem.
Low / mid class mobile devices are struggling. The window in chro…
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Dear Velocyto team,
I have a question about running velocyto on eight 10x samples. Since 10X pipeline gives bam file for each sample, I can run velocyto on each bam file one by one and generate eig…
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Dear Professor,
I am very impressed with the STitch3D technology. I want to know how to do the model evaluation with different methods. I think the data processing part is different among differe…
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Hi, with the help of Rob I managed to get a Rust BD Rhapsody analysis tool to work (https://github.com/stela2502/Rustody).
It supports all versions of BD's beads at the moment, but not the (new) whol…